DNA isolation / purification Cells Immortalized cell lines

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Get tips on using gRNA_Cloning Vector to perform CRISPR Mouse - Deletion RMA cells Trh4

Products Addgene gRNA_Cloning Vector

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates MCF-7 human breast cancer

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates NHEK normal human epidermal keratinocytes
celecoxib Product

Get tips on using celecoxib to perform Autophagy assay cell type - U87MG

Products pfizer celecoxib

Get tips on using Corning® BioCoat™ Matrigel® Invasion Chamber with 8.0 µm PET Membrane in four 6-well Plates to perform Cell migration / Invasion cell type - 4T1

Products Corning Corning® BioCoat™ Matrigel® Invasion Chamber with 8.0 µm PET Membrane in four 6-well Plates

Get tips on using MultiTox-Fluor Multiplex Cytotoxicity Assay to perform Live / Dead assay mammalian cells - RAW 264.7

Products Promega MultiTox-Fluor Multiplex Cytotoxicity Assay

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay mammalian cells - THP-1

Products Merck Millipore Live/Dead Double Staining Kit (Merck)

DNA PCR Quantitative real-time PCR

DNA PCR Conventional / Qualitative PCR V

DNA PCR Conventional / Qualitative PCR V

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