rna-isolation-purification-cells-immortalized-cal-27

Get tips on using MycoTOOL Mycoplasma Real-Time PCR Kit to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using Ion AmpliSeq™ Cancer Hotspot Panel v2 to perform Cell line authentication Lung carcinoma A549 cell line

Get tips on using Rat Endothelial Cell Growth Medium to perform Mammalian cell culture media RAOEC

Get tips on using MicroSEQ™ D2 rDNA Fungal PCR Kit to perform Cell Culture Contamination Detection Kit Fungi

Get tips on using Fast MicroSEQ™ D2 Fungal rDNA PCR Kit to perform Cell Culture Contamination Detection Kit Fungi

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using NA-Star™ Influenza Neuraminidase Inhibitor Resistance Detection Reagent Set to perform Cell Culture Contamination Detection Kit Virus

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer

Get tips on using LSI VetMAX™ Bluetongue Virus Real-Time PCR Kit, BTV1 typing - IAH to perform Cell Culture Contamination Detection Kit Virus

Get tips on using Endothelial Cell Medium to perform Mammalian cell culture media CADMEC/HMVEC