ChIP H3K36Me3 Canine Human

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Mouse - 3T3-L1 cells

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 to perform ChIP Rat - Mesenteric arteries

Get tips on using Human Syndecan-1 ELISA Kit (CD138) (ab46506) to perform ELISA Human - SDC1

Get tips on using Human NRG1 beta 1 ELISA Kit (ab100614) to perform ELISA Human - NRG1

Get tips on using Human CCL2/MCP-1 Quantikine ELISA Kit to perform ELISA Human - MCP1

Get tips on using Human MPO/Myeloperoxidase PicoKine™ ELISA Kit to perform ELISA Human - MPO

Get tips on using Human EGFR In-Cell ELISA Kit (ab126419) to perform ELISA Human - EGFR

Get tips on using Pan Monocyte Isolation Kit, human to perform Cell Isolation Monocyte

Get tips on using Classical Monocyte Isolation Kit, human to perform Cell Isolation Monocyte