Protein Expression Prokaryotic cells C. glutamicum

Get tips on using VetMAX™ C. fetus Kit to perform Cell Culture Contamination Detection Kit Bacteria

Get tips on using Notch 1 Antibody (C-20) to perform Autophagy assay cell type - HEK 293

Get tips on using cytochrome c Antibody (A-8): sc-13156 to perform Western blotting Cytochrome C

Get tips on using Recombinant Anti-Cytochrome C antibody [EPR1327] (ab133504) to perform Western blotting Cytochrome C

Get tips on using Rat/Mouse Cytochrome c Quantikine ELISA Kit to perform ELISA Mouse - Cytochrome c

Get tips on using Rat/Mouse Cytochrome c Quantikine ELISA Kit to perform ELISA Rat - Cytochrome c

Get tips on using Recombinant Anti-c-Jun antibody [E254] (ab32137) to perform Western blotting C-Jun

Get tips on using Qproteome Mammalian Protein Prep Kit to perform Protein isolation Tissue - Human tissue C-MFPE samples

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using APC anti-mouse CD117 (c-kit) Antibody to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit