RNA isolation / purification Cells primary

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat dermal fibroblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat cortical neurons

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat cardiac fibroblasts

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary rat lymph node cells

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary lamb pulmonary artery endothelial cells