protein-isolation-tissue-mouse-lung-tissue

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using High Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Wide-Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Human myogenic progenitor cells

Get tips on using Fluorescent High Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Human neural progenitor cells (NPC)

Get tips on using STEMdiff™ Hematopoietic Kit to perform Stem cell Differentiation media hiPSCs differentiation into CD43+ primitive hematopoietic progenitor cells (HPCs)

Get tips on using Gibco™Ham's F-12K (Kaighn's) Medium to perform Stem cell culture media Human myogenic progenitor cells

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - Islets of langerhans (Beta cells)

Get tips on using ApoAlert™ DNA Fragmentation Assay Kit to perform TUNEL assay cell type - Islets of langerhans (Beta cells)