A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.
Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Colon cancer cell line LS-174T
Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Colon cancer cell line DLD-1
Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Colon cancer cell line NCI-H508
Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Colon cancer cell line HCT-15
Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Colon cancer cell line HCT-116
Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line SW480
Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line SW620
Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line RKO
Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line LoVo
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment