Protein Expression Prokaryotic cells R. erythropolis

Get tips on using ON-TARGETplus Rat Tp53 (24842) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - C6 (rat glioma) p53

Get tips on using Orai1 Rat siRNA Oligo Duplex (Locus ID 304496) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Orai1

Get tips on using Recombinant Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) to perform ChIP Anti-bodies ERα

Get tips on using ON-TARGETplus Rat Snap23 (64630) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Snap23

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Rat - Point mutation Rat-2 MMP-9 promoter

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Rat - PC12

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - RAW264.7