siRNA / miRNA gene silencing Human MDA-MB-453

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using CHO Media to perform Mammalian cell culture media CHO

Get tips on using HEK GM to perform Mammalian cell culture media HEK

Get tips on using pSVA1470 to perform Protein Expression Prokaryotic cells - S. acidocaldarius MalR

Get tips on using pSVA937 to perform Protein Expression Prokaryotic cells - E. coli MalR

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Rat_Liver

Get tips on using Medium 199 to perform Mammalian cell culture media PAOSMC

Get tips on using Advanced DMEM to perform Mammalian cell culture media HC

Get tips on using Medium 231 to perform Mammalian cell culture media HAOSMC

Get tips on using Medium 200 to perform Mammalian cell culture media HAOECv