Protein Expression Prokaryotic cells R. erythropolis

Get tips on using XbaI R6181 to perform Restriction Enzymes XbaI

Get tips on using SmaI R6121 to perform Restriction Enzymes SmaI

Get tips on using BamHI R6025 to perform Restriction Enzymes BamHI

Get tips on using HindIII R6041 to perform Restriction Enzymes HindIII

Get tips on using RAC1 Antibody to perform Western blotting Rac1

Get tips on using DdeI R6291 to perform Restriction Enzymes DdeI / HpyF3I

Get tips on using HaeIII R6171 to perform Restriction Enzymes HaeIII / BsuRI

Get tips on using HpaII R6311 to perform Restriction Enzymes HpaII / MspI

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.