Immunohistochemistry Collagen Type III

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using GR Antibody (G-5): sc-393232 to perform Immunohistochemistry Human - GR/glucocorticoid receptor

Get tips on using PDGF Receptor β (28E1) Rabbit mAb to perform Immunohistochemistry PDGFβR - Rabbit Mouse -NA-

Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform Immunohistochemistry γH2AX - Mouse Human -NA-

Get tips on using Anti-pan Cytokeratin antibody [C-11] (ab7753) to perform Immunohistochemistry Human - pan Cytokeratin

Get tips on using β-catenin Antibody (E-5): sc-7963 to perform Immunohistochemistry Human - β-catenin

Get tips on using anti-Mucin 6, Clone: CLH5, Invitrogen™ to perform Immunohistochemistry Human - MUC-6

Get tips on using MUC5AC Antibody / Mucin-5AC [clone 45M1] (V2198) to perform Immunohistochemistry Human - Muc-5AC

Get tips on using Mucin 2 Antibody (F-2): sc-515032 to perform Immunohistochemistry Human - Muc-2