Protein Expression Prokaryotic cells B. licheniformis

Get tips on using FAS Antibody (B-10): sc-8009 to perform Western blotting Fas

Get tips on using Akt1 Antibody (B-1): sc-5298 to perform Western blotting AKT

Get tips on using IRE1α Antibody (B-12): sc-390960 to perform Immunohistochemistry Mouse - IRE1α

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using IKKα Antibody (B-8): sc-7606 to perform Western blotting IKK Alpha

Get tips on using EpiCult™-B Mouse Medium Kit to perform Stem cell culture media Murine mammospheres

Get tips on using CC10 Antibody (B-6): sc-390313 to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using ALCAM Antibody (B-6): sc-74558 to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using COL3A1 Antibody (B-10): sc-271249 to perform Western blotting Type III collagen

Get tips on using Granzyme B Monoclonal Antibody (NGZB), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - Granzyme B