Protein Expression Prokaryotic cells B. subtilis

Get tips on using EasySep™ Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell

Get tips on using MACSprep™ HLA B Cell Isolation Kit, human to perform Cell Isolation HLA B Cell

Get tips on using JAK1 Antibody (B-3): sc-376996 to perform Western blotting Jak1

Get tips on using Smad4 Antibody (B-8): sc-7966 to perform Western blotting Smad4

Get tips on using Cdc42 Antibody (B-8): sc-8401 to perform Western blotting Cdc42

Get tips on using FAS Antibody (B-10): sc-8009 to perform Western blotting Fas

Get tips on using Akt1 Antibody (B-1): sc-5298 to perform Western blotting AKT

Get tips on using IRE1α Antibody (B-12): sc-390960 to perform Immunohistochemistry Mouse - IRE1α

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using IKKα Antibody (B-8): sc-7606 to perform Western blotting IKK Alpha