Protein Expression Prokaryotic cells C. glutamicum

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using Cytochrome c Releasing Apoptosis Assay Kit to perform Apoptosis assay cell type - SKOV3

Get tips on using VWF Antibody (C-12): sc-365712 to perform Immunohistochemistry Mouse - vWF

Get tips on using VEGF Antibody (C-1): sc-7269 to perform Immunohistochemistry Mouse - VEGFA

Get tips on using Tak1 Antibody (C-9): sc-7967 to perform Western blotting Tak1

Get tips on using VEGF Antibody (C-1): sc-7269 to perform Western blotting VEGF

Get tips on using JAK2 Antibody (C-10): sc-390539 to perform Western blotting Jak2

Get tips on using p-c-Jun Antibody (KM-1): sc-822 to perform Western blotting C-Jun

Get tips on using PARP-1 C-terminal antibody (pAb) to perform ChIP Anti-bodies PARP

Get tips on using Stat5b Antibody (C-8): sc-377069 to perform ChIP Anti-bodies Stat5b