Protein Expression Prokaryotic cells E. coli

Get tips on using QIAGEN Large-Construct Kit to perform Plasmid Isolation Shiga toxin-producing E. coli

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using EasySelect™ Pichia Expression Kit to perform Protein expression and purification Yeast - Pichia pastoris Chymase

Get tips on using Rat soluble E-selectin,sE-selectin ELISA Kit to perform ELISA Rat - sE-Selectin

Get tips on using E-Gel™ Ultra Low Range DNA Ladder to perform DNA Ladder Low Range

Get tips on using E-Gel™ Low Range Quantitative DNA Ladder to perform DNA Ladder Low Range

Get tips on using E-Gel™ 1 Kb Plus DNA Ladder to perform DNA Ladder 1 kb

Get tips on using pCEP4 Mammalian Expression Vector to perform Protein expression and purification Bacteria - Origami™ B (DE3) ABCA4

Get tips on using pEGFP-INHα to perform Protein Expression Eukaryotic cells - BHK cells INHα

Get tips on using pFastBac™ Dual Expression Vector to perform Protein expression and purification Bacteria - DH10Bac™ GYS1-GN1