rna-isolation-purification-cells-immortalized-cal-27

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - THP-1

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - MCF-7

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - SH-SY5Y

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Human cancer esophageal organoids

Get tips on using MCDB 131 Medium to perform Mammalian cell culture media CADMEC/HMVEC

Get tips on using Goat antibody to Calretinin to perform Immunohistochemistry Mouse - Calb2

Get tips on using MarkerGene™ Live Cell Fluorescent Reactive Oxygen Species Detection Kit to perform ROS assay cell type - T47D

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human gastric cancer organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human liver cancer organoids

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.