siRNA / miRNA gene silencing Human MDA-MB-468

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MARC-nsp11

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MARC-145

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Ma-Mel

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MA-104

Get tips on using Targefect-HUVEC to perform DNA transfection Mammalian cells - Primary cells HUVEC

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines PANC-1

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A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Rat_Renal tissue

Get tips on using TRI Reagent® to perform Protein isolation Mammalian cells - Mouse_Brown fat