DNA gel extraction / PCR product purification

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using EpiCult™-B Mouse Medium Kit to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using DMEM/F-12, no phenol red to perform 3D Cell Culture Media Mouse primary lung epithelial cells-organoids

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human primary breast ephitelial cells-organoids

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

Get tips on using pFastBac1-A/reassortant/NYMC X-179-NP to perform Protein Expression Eukaryotic cells - S. frugiperda Influenza NP

Get tips on using Gibco™Ham's F-12K (Kaighn's) Medium to perform Stem cell culture media Human myogenic progenitor cells

Get tips on using VWR Life Science RIPA Lysis Buffer, Biotechnology Grade to perform Protein isolation Mammalian cells - Caco-2

Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - Point mutation L929 caspid protein

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - THP-1