Protein Expression Eukaryotic cells CHO

Get tips on using Goat Anti-Type III Collagen-BIOT to perform Immunohistochemistry Collagen Type III - Goat Mouse Biotin

Get tips on using Quick Amp Labeling Kit-one color to perform Microarray RNA amplification & Labeling - Mouse cochlaea Cyanine CTP

Get tips on using Negative control, native pGL4.13 luciferase vector to perform Reporter gene assay luciferase - negative control (luciferase vector)

Get tips on using Quick Amp Labeling Kit-one color to perform RNA amplification & labeling Mammalian - RNA, Mice Cochlaea Cyanine CTP

Get tips on using Monoclonal Anti-Collagen, Type III antibody produced in mouse to perform Western blotting Type III collagen

Get tips on using EZ-10 Spin Column Plasmid DNA Miniprep Kit to perform Plasmid Isolation E. coli-S. cerevisiae transconjugate

Get tips on using Anti Type X Collagen (raised against rat) pAb (Rabbit, Antiserum) to perform Immunohistochemistry Mouse - Col X

Get tips on using Silencer® Select Negative Control No 1 siRNA to perform siRNA / miRNA gene silencing Human - siRNA negative control Lipid

Get tips on using Silencer® Select Negative Control No 1 siRNA to perform siRNA / miRNA gene silencing Mouse - siRNA negative control polymer / lipid

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.