rna-isolation-purification-cells-immortalized-brl-3a

Get tips on using BclI (10 U/µL) to perform Restriction Enzymes BclI

Get tips on using BglI (10 U/µL) to perform Restriction Enzymes BglI

Get tips on using Human/Mouse/Rat SOX2 Antibody to perform Western blotting SOX2

Get tips on using mTOR (7C10) Rabbit mAb #2983 to perform Western blotting mTOR

Get tips on using BseLI (BslI) (10 U/µL) to perform Restriction Enzymes BseLI / BslI

Get tips on using TIMP2 (D18B7) Rabbit mAb #5738 to perform Western blotting TIMP-2

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Anti-TAU antibody produced in rabbit to perform Western blotting Tau

Get tips on using HER2/ErbB2 (29D8) Rabbit mAb #2165 to perform Western blotting HER2