Acid phosphatase assay cell type

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RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA RNA sequencing Human Glioblastoma stem-like cells (GSCs)

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA RNA sequencing Mouse ESCs (Embryonic Stem Cells)

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA RNA sequencing Rat Retinal ganglion cells (RGCs)

Get tips on using pPIC9-His-FIP-Ganoderma to perform Protein Expression Eukaryotic cells - P. pastoris Ganoderma FIP

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Get tips on using pTip-QC2-gi_21221697 to perform Protein Expression Prokaryotic cells - R. erythropolis putative gntR-family regulator

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Get tips on using pTip-QC2-gi_21218674 to perform Protein Expression Prokaryotic cells - R. erythropolis putative DNA-binding protein

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Get tips on using pMHL_2002-bfloGFPa1 to perform Protein Expression Eukaryotic cells - T. pseudonana IbpA DR2 antigen from Histophilus somni

Products Mark Hildebrand, Marine Biology Research Division, Scripps Insti pMHL_2002-bfloGFPa1

Get tips on using pSS1-EBA140RIII–V to perform Protein Expression Prokaryotic cells - L. lactis EBA140RIII–V P. falciparum

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Get tips on using pIEX-5-6His-mMBP-UMODpXR to perform Protein Expression Eukaryotic cells - HEK293 mMBP

Products Luca Jovine, Karolinska Institutet, Department of Biosciences an pIEX-5-6His-mMBP-UMODpXR

Get tips on using MrNV-pGEX-6P-1 to perform Protein Expression Prokaryotic cells - E. coli MrNV capsid

Products Paisarn Sithigorngul, Department of Biology, Faculty of Science, MrNV-pGEX-6P-1

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