rna-isolation-purification-cells-primary-rat-cortical-neurons

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - HeLa

Get tips on using Ki67 antigen rabbit polyclonal antibody to perform Cell cytotoxicity / Proliferation assay cell type - malignant peripheral nerve sheath tumor

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - THP-1

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - HEK 293

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - SH-SY5Y

Get tips on using LAMP2 (D5C2P) Rabbit mAb to perform Autophagy assay cell type - GH3

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - HaCaT

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - A549

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - HeLa