When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
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I work with Human liver organoids and I would like to know the impact of FBS on the organoids. Since the composition of FBS is unknown, would you recommend any alternatives such as human platelet lysate?
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