Site Directed Mutagenesis (SDM) Mouse Deletion C2C12

Get tips on using Mouse Fibronectin PicoKine™ ELISA Kit to perform ELISA Mouse - Fibronectin

Get tips on using Mouse Decorin ELISA Kit (DCN) (ab155454) to perform ELISA Mouse - Decorin

Get tips on using Mouse BDNF PicoKine™ ELISA Kit to perform ELISA Mouse - BDNF

Get tips on using Mouse TNF alpha ELISA Kit (ab208348) to perform ELISA Mouse - TNF-alpha

Get tips on using Mouse TNF-alpha Quantikine ELISA Kit to perform ELISA Mouse - TNF-alpha

Get tips on using Mouse RANKL ELISA Kit (TNFSF11) (ab100749) to perform ELISA Mouse - RANK L

Get tips on using Mouse Lipocalin-2/NGAL DuoSet ELISA to perform ELISA Mouse - NGAL/LCN2

Get tips on using Mouse Dkk-1 Quantikine ELISA Kit to perform ELISA Mouse - Dkk-1

Get tips on using ScriptSeq Complete Kit (Human/Mouse/Rat) to perform RNA sequencing Mouse - J774

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.