rna-isolation-purification-cells-primary-rat-brain-microvascular-endothelial-cells

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.

Get tips on using EBMTM Endothelial Cell Growth Basal Medium, 500 mL to perform 3D Cell Culture Media Human blood-brain barrier organoid

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Brain

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood monocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human fibroblast-like synoviocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes - rheumatoid arthritis

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human osteoblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine monocyte derived macrophages