DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using YFP‐TOP2β to perform Protein Expression Eukaryotic cells - HEK293 TOP2β

Get tips on using YFP‐TOP2α to perform Protein Expression Eukaryotic cells - HEK293 TOP2α

Get tips on using pcDNA-nGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1

Get tips on using pcDNA-oGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1

Get tips on using pMT3-RhoGC to perform Protein Expression Eukaryotic cells - HEK293 RhoGC

Get tips on using pONE-40A to perform Protein Expression Eukaryotic cells - HEK293 MBP

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized HepG2

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform 3D Cell Culture Media Primary human breast tumors-Mammospheres

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell culture media Brain organoids from Human iPSCs

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.