rna-isolation-purification-cells-primary-mouse-cortical-neurons

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HK-2

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - HK-2

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - CAKI-1

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HEK 293

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Human - Kupffer Cells

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - human umbelical vein endothelial cells (HUVEC)

Get tips on using HIF-1α siRNA (r) to perform siRNA / miRNA gene silencing Rat - Brain endothelial cells HIF-1α Lipid

Get tips on using C/EBP β siRNA (r) to perform siRNA / miRNA gene silencing Rat - Glial cells C/EBP‐β

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231

What is the optimal concentration for primers in qPCR? My total volume is 20μl per reaction.