rna-isolation-purification-cells-immortalized-mda-mb-231

Get tips on using Unstained Protein Ladder, Broad Range Ladder to perform Protein Ladder Unstained

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using PageRuler™ Unstained High Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained Broad Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained Low Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using Spectra™ Multicolor High Range Protein Ladder to perform Protein Ladder Prestained

Get tips on using Spectra™ Multicolor Low Range Protein Ladder to perform Protein Ladder Prestained

Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Hamster - Point mutation BHK-21 PI4KA

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.