rna-isolation-purification-cells-immortalized-mda-mb-231

- Found 9133 results

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Hamster - Point mutation BHK-21 LCMV (lymphocytic choriomeningitis virus)

Products Thermo Fisher Scientific Phusion Site-Directed Mutagenesis Kit

Get tips on using Unstained Protein Standard, Broad Range (10-200 kDa) to perform Protein Ladder Unstained

Products New England BioLabs Unstained Protein Standard, Broad Range (10-200 kDa)

Get tips on using Color Prestained Protein Standard, Broad Range (10-250 kDa) to perform Protein Ladder Prestained

Products New England BioLabs Color Prestained Protein Standard, Broad Range (10-250 kDa)

Get tips on using Blue Prestained Protein Standard, Broad Range (11-250 kDa) to perform Protein Ladder Prestained

Products New England BioLabs Blue Prestained Protein Standard, Broad Range (11-250 kDa)

Get tips on using Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027) to perform Protein Ladder Prestained

Products Abcam Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)

Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Hamster - Point mutation BHK-21 DENV4

Products Agilent Technologies QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Gene specific profiling RH30 rhabdomyosarcoma IGF2

Get tips on using GenLadder 100 bp + 1.5 kbp (ready-to-use), DNA marker to perform DNA Ladder 100 bp

Products Genaxxon bioscience GenLadder 100 bp + 1.5 kbp (ready-to-use), DNA marker

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Proteins Western blotting Ras

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Proteins Western blotting Rac1

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