siRNA / miRNA gene silencing Human MEG-01

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Get tips on using AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2 to perform PCR Multiplex PCR - Mammalian DNA

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - MG-63

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MG63

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized MG63

Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Cells - immortalized MG63

Get tips on using Apoptotic DNA Ladder Isolation Kit to perform DNA Damage Assay U-87 MG

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - MG-63

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - MG-63

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay U-87 MG