Protein Expression Prokaryotic cells C. crescentus JS1014

Get tips on using Lamin B1 Antibody (C-5): sc-365962 to perform Western blotting Lamin B

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - SK-N-BE(2)-C

Get tips on using PDGFR-α Antibody (C-9): sc-398206 to perform Flow cytometry Anti-bodies Human - CD140/PDFGR2

Get tips on using CpGfree to perform Protein Expression Eukaryotic cells - CHO EGFP

Get tips on using CpGrich to perform Protein Expression Eukaryotic cells - CHO EGFP

Get tips on using PL_MmXbp1s to perform Protein Expression Eukaryotic cells - CHO Xbp1s

Get tips on using PL_MmPdia3 to perform Protein Expression Eukaryotic cells - CHO Pdia3

Get tips on using PL_MmHspa5 to perform Protein Expression Eukaryotic cells - CHO Hspa5