shRNA gene silencing Mouse RGC-5

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using T4 DNA Ligase (5 U/µL) to perform DNA ligation

Get tips on using Vimentin Antibody (E-5): sc-373717 to perform Western blotting Vimentin

Get tips on using Anti-SMAD1/5/9 antibody (ab66737) to perform Western blotting Smad1

Get tips on using p21 Antibody (F-5): sc-6246 to perform Western blotting p21

Get tips on using Lamin B1 Monoclonal Antibody (L-5) to perform Western blotting Lamin B

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA methylation profiling Whole genome profiling - human germ cell cancer

Get tips on using β-catenin Antibody (E-5): sc-7963 to perform Immunohistochemistry Human - β-catenin

Get tips on using ICAM-1 Antibody (G-5): sc-8439 to perform Western blotting ICAM-1