The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.
The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to be considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.
Get tips on using ON-TARGETplus Human NOS2 (4843) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Caco-2 iNOS/NOS2
Get tips on using ON-TARGETplus Human CFL1 (1072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 CFL1
Get tips on using ON-TARGETplus Human NBN (4683) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MCF-7 NBS1/NBN
Get tips on using ON-TARGETplus Human RAD51 (5888) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 RAD51
Get tips on using ON-TARGETplus Human AHR (196) siRNA - Individual to perform siRNA / miRNA gene silencing Human - MDA-MB-231 AHR
Get tips on using ON-TARGETplus Human PCSK6 (5046) siRNA - Individual to perform siRNA / miRNA gene silencing Human - Detroit 562 / D562 PACE4
Get tips on using ON-TARGETplus Human FURIN (5045) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Detroit 562 / D562 Furin
Get tips on using ON-TARGETplus Human PCSK7 (9159) siRNA - Individual to perform siRNA / miRNA gene silencing Human - Detroit 562 / D562 PC7
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