siRNA / miRNA gene silencing Rat Cardiomyocyte (H9C2) HIF-1α

Get tips on using Cnp siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 CNPase

Get tips on using Wwtr1 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 Taz

Get tips on using Bambi siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 BAMBI

Get tips on using Stk11 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 Stk11

Get tips on using Cxcr4 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 CXCR4

Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - BMDMs Dusp3

Get tips on using PACE4 siRNA (h) to perform siRNA / miRNA gene silencing Human - DuCaP

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using ADAMTS13 siRNA to perform siRNA / miRNA gene silencing Human - HUVEC ADAMTS-13

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - LuCaP-77 SKIL