DNA isolation / purification Cells Immortalized cell lines

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media DAOY

Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)

Get tips on using pTSara-NatB to perform Protein Expression Prokaryotic cells - E. coli N-terminal acetyltransferase B

Get tips on using pQE-30 to perform Protein Expression Prokaryotic cells - E. coli Guinea Pig TNF-Alpha

Get tips on using pET30a-β4 to perform Protein Expression Prokaryotic cells - E. coli E. granulosus β4 tubulin

Get tips on using pET30a-α9 to perform Protein Expression Prokaryotic cells - E. coli E. granulosus α9 tubulin

Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

Get tips on using pFastBac-MRP4-his6 to perform Protein Expression Eukaryotic cells - S. frugiperda human MRP4-his6