DNA Damage Assay Human bronchial epithelial cells (hBE)

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - Caco-2

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - SH-SY5Y

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - BHK-21

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using pGEX-FIP-nha to perform Protein Expression Prokaryotic cells - E. coli FIP Nectria haematococca

Get tips on using PL_MmXbp1s to perform Protein Expression Eukaryotic cells - CHO Xbp1s

Get tips on using PL_MmPdia3 to perform Protein Expression Eukaryotic cells - CHO Pdia3

Get tips on using PL_MmHspa5 to perform Protein Expression Eukaryotic cells - CHO Hspa5

Get tips on using PL_MmHsp90b1 to perform Protein Expression Eukaryotic cells - CHO Hsp90b1