The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells to perform Reporter gene assay β-galactosidase substrates - BHK-21 baby hamster kidney cells
Get tips on using QIAfilter Plasmid Kits to perform Plasmid Isolation Enterobacteriaceae
Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Stenotrophomonas maltophilia
Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Bacteroides xylanisolvens
Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Serratia marcescens
Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Bacteroides xylanisolvens
Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Klebsiella pneumoniae
Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Enterobacter cloacae
Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Aeromonas spp.
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment