Protein expression and purification Insect cells Hi5

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Get tips on using LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation to perform Live / Dead assay mammalian cells - rat testicular tissue

Products Thermo Fisher Scientific LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Discussions Problem in phase separation after using serum/plasma kit

Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform TissueFAxs phospho-Histone H2A.X (Ser139) - Mouse Human

Products Millipore Anti-phospho-Histone H2A.X (Ser139) Antibody

Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform Immunofluorscence  phospho-Histone H2A.X (Ser139) - Mouse Human

Products Millipore Anti-phospho-Histone H2A.X (Ser139) Antibody

Get tips on using Anti-HIF-1 alpha antibody - ChIP Grade (ab2185) to perform Western blotting HIF-1 alpha

Products Abcam Anti-HIF-1 alpha antibody - ChIP Grade (ab2185)

Get tips on using QIAGEN Protease (30 AU) to perform Digestion during DNA preparation

Products Qiagen QIAGEN Protease (30 AU)

Get tips on using HIF-1 alpha Antibody to perform Western blotting HIF-1 alpha

Products Novus Biologicals HIF-1 alpha Antibody

Get tips on using HIF-1α Antibody (28b): sc-13515 to perform Western blotting HIF-1 alpha

Products Santa Cruz Biotechnology HIF-1α Antibody (28b): sc-13515

Get tips on using Anti-trimethyl-Histone H3 (Lys9) Antibody to perform ChIP Anti-bodies H3K9me3

Products Millipore Anti-trimethyl-Histone H3 (Lys9) Antibody

Get tips on using Anti-trimethyl-Histone H3 (Lys27) Antibody to perform ChIP Anti-bodies H3K27me3

Products Millipore Anti-trimethyl-Histone H3 (Lys27) Antibody

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