siRNA / miRNA gene silencing Rat Brain endothelial cells HIF-1α

Get tips on using TAGZyme Qcyclase/pGAPase Enzymes (150 U) to perform Protein tag His-tag removal

Get tips on using RGS·His Antibody, BSA-free (100ug) to perform Protein tag Detection of His-tagged proteins

Get tips on using Penta·His Alexa Fluor 488 Conjugate to perform Protein tag Detection of His-tagged proteins

Get tips on using Type-it HRM PCR Kit (2000) to perform High-resolution melting (HRM) analysis Bacterial

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - RAW264.7

Get tips on using Ni-NTA Fast Start Kit (6) to perform Protein tag Purification of His-tagged proteins

Get tips on using Penta·His Antibody, BSA-free (100 ug) to perform Protein tag Detection of His-tagged proteins

Get tips on using Tetra·His Antibody, BSA-free (100 µg) to perform Protein tag Detection of His-tagged proteins

Get tips on using Ni-NTA Superflow 96 BioRobot Kit (4) to perform Protein tag Purification of His-tagged proteins

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.