rna-isolation-purification-cells-immortalized-cal-27

Get tips on using Carboxy-H2DCFDA (general oxidative stress indicator) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma

Get tips on using PowerPlex® 16 HS System to perform Cell line authentication Breast carcinoma cell line BT474

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay-Silver to perform DNA Damage Assay Caco-2

Get tips on using RPMI 1640 to perform Mammalian cell culture media CML T1

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - BxPc-3 human primary pancreatic adenocarcinoma

Get tips on using Ion AmpliSeq™ Sample ID Panel to perform Cell line authentication Lung carcinoma A549 cell line

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using 96-Well Cell Invasion Assay, Basement Membrane to perform Cell migration / Invasion cell type - RAW 264.7

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Endometrial Cancer cell line KLE

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Endometrial Cancer cell line Ishikawa