Protein Expression Eukaryotic cells S. cerevisiae

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Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Mouse - Neuroblastoma 2a dibasic site, R18Q

Products Agilent Technologies QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction

DNA PCR Hot start PCR Mammalian DNA

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA PCR Methylation specific PCR Bacterial DNA

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA PCR Methylation specific PCR Mammalian DNA

Get tips on using MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles to perform shRNA gene silencing Human - Islets of langerhans Negative control (scrambled) lentiviral particles

Products Sigma-Aldrich MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles

DNA DNA isolation / purification Soil samples

Get tips on using FastDigest SdaI to perform Restriction Enzymes SbfI / SdaI

Products Thermo Fisher Scientific FastDigest SdaI

RNA siRNA / miRNA gene silencing Mouse BV2 LGAL3S3

RNA siRNA / miRNA gene silencing Rat PC12 chorein

RNA siRNA / miRNA gene silencing Rat PC12 Fabp5

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