siRNA / miRNA gene silencing Rat MTLn3 (rat mammary adenocarcinoma breast cancer cell line)

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Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Mouse - RAW264.7

Products Thermo Fisher Scientific Pierce™ Agarose ChIP Kit

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Mouse - RAW264.7

Products Sigma-Aldrich Imprint® Chromatin Immunoprecipitation Kit

Get tips on using Anti-Ki-67 Antibody to perform Immunohistochemistry Ki67 - Rabbit Mouse -NA-

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Get tips on using Low Molecular Weight DNA Ladder to perform DNA Ladder Low Range

Products New England BioLabs Low Molecular Weight DNA Ladder

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Activation RANKL

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Get tips on using Anti-acetyl-Histone H3 Antibody to perform ChIP acH3 - Rabbit Human YFP

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Get tips on using 53BP1 Antibody (H-300) to perform TissueFAxs 53BP1 [H-300] - Rabbit Human -NA-

Products Santa Cruz Biotechnology 53BP1 Antibody (H-300)

Get tips on using 53BP1 Antibody (H-300) to perform Immunofluorscence  53BP1 [H-300] - Rabbit Human -NA-

Products Santa Cruz Biotechnology 53BP1 Antibody (H-300)

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

Cellular assays Necrosis Caco-2

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

Cellular assays Necrosis HCT 116

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