Immunohistochemistry Collagen VII antibody [LH7.2] Mouse

Get tips on using SQSTM1/p62 Antibody to perform Autophagy assay cell type - THP 1

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Vimentin Antibody (E-5): sc-373717 to perform Western blotting Vimentin

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using SQSTM1/p62 Antibody to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using Alexa Fluor® 700 Mouse Anti-Mouse NK1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1

Get tips on using ATP5A1 Monoclonal Antibody (7H10BD4F9) to perform Western blotting ATP5A

Get tips on using TBP Monoclonal Antibody (1TBP18) to perform Western blotting TBP

Get tips on using Anti-NLRP3 antibody (ab214185) to perform Western blotting NLRP3

Get tips on using FOXP3 Rabbit Polyclonal Antibody to perform Western blotting FOXP3