Immunohistochemistry chk2 phospho (Thr 68) Rabbit IgG Human

Get tips on using VWF Antibody (C-12): sc-365712 to perform Immunohistochemistry Mouse - vWF

Get tips on using VEGF Antibody (C-1): sc-7269 to perform Immunohistochemistry Mouse - VEGFA

Get tips on using Anti-Peptide YY/PYY antibody (ab131246) to perform Immunohistochemistry Mouse - PYY

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using ON-TARGETplus Human CDK2 (1017) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CDK2

Get tips on using Recombinant Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917) to perform Immunohistochemistry Mouse - VEGFA

Get tips on using Anti-Mouse CD31 (PECAM-1) to perform Immunohistochemistry CD31 - Rat Mouse -NA-

Get tips on using Anti-NF-kB p65 antibody (ab16502) to perform Immunohistochemistry Mouse - NFκB / p65

Get tips on using Mucin 2 Antibody (Ccp58): sc-7314 to perform Immunohistochemistry Mouse - Muc-2

Get tips on using ALCAM Antibody (B-6): sc-74558 to perform Immunohistochemistry Mouse - CD166 / ALCAM