pcr-multiplex-pcr-mammalian-dna

Get tips on using ICAfectin®442 siRNA transfection to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HepG2

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using Bone DNA Purification Kit to perform DNA isolation / purification Tissue - bone

Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Tissue - bone

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Tissue - bone

Get tips on using QIAamp DNA Micro Kit to perform DNA isolation / purification Tissue - adipose

Get tips on using T4 DNA Ligase, Cloned to perform DNA ligation

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.