siRNA / RNAi /miRNA transfection Human Cells HeLa

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay mouse - 3T3-L1

Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Rat - Heart

Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Rat - Heart

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - heart

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Rat - Heart

Get tips on using Ad-Sal-shRNA to perform shRNA gene silencing Rat - H9c2 salusin-β

Get tips on using Ad-Sal-shRNA to perform shRNA gene silencing Rat - WKY Salusin-β

Get tips on using Individual: TRC Mouse Cdh1 shRNA to perform shRNA gene silencing Mouse - 4T1 Cdh1

Get tips on using IL-4Rα shRNA (m) Lentiviral Particles to perform shRNA gene silencing Mouse - R221a IL4Rα

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).