RNA isolation / purification Tissue Human

Get tips on using Rn_Ywhaz_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - H9c2 14-3-3 f/Ywhaz

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - Raw 264.7

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - rat plasma

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using High Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Low Range DNA Ladder to perform DNA Ladder Low Range

Get tips on using Mid Range DNA Ladder to perform DNA Ladder Medium Range

Get tips on using Wide-Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Medium Range DNA Ruler to perform DNA Ladder Medium Range

Get tips on using Fluorescent High Range DNA Ladder to perform DNA Ladder Long Range