Get tips on using SQSTM1/p62 Antibody to perform Autophagy assay cell type - U937
Get tips on using Beclin 1 Antibody to perform Autophagy assay cell type - A2780
Get tips on using SQSTM1/p62 Antibody to perform Autophagy assay cell type - A2780
Get tips on using LC3A/B Antibody to perform Autophagy assay cell type - A2780
Get tips on using Anti-LC3 Antibody to perform Autophagy assay cell type - MCF7
Get tips on using LC3A/B Antibody to perform Autophagy assay cell type - MCF7
Get tips on using Anti-mTOR antibody to perform Autophagy assay cell type - HepG2
Get tips on using Anti-LC3 pAb to perform Autophagy assay cell type - A549
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
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