CRISPR Mouse Deletion RMA cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Monoclonal Mouse Anti-Bromodeoxyuridine (Concentrate) Clone Bu20a to perform Immunohistochemistry Mouse - BrdU

Get tips on using Mouse OPN / Osteopontin PicoKine™ ELISA Kit to perform ELISA Mouse - OPN

Get tips on using Mouse VEGF PicoKine™ Fast ELISA Kit to perform ELISA Mouse - VEGF

Get tips on using Mouse Total MMP-9 Quantikine ELISA Kit to perform ELISA Mouse - MMP9

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay .

Get tips on using Human/Mouse NLRP3/NALP3 Antibody to perform Western blotting NLRP3

Get tips on using PCNA (PC10) Mouse mAb #2586 to perform Western blotting PCNA

Get tips on using Mouse GDNF ELISA Kit (ab171178) to perform ELISA Human - GDNF

Get tips on using Human/Mouse BDNF DuoSet ELISA to perform ELISA Human - BDNF