shRNA gene silencing Human THP-1

Get tips on using pSUPER.retro.neo+gfp vector- Syn G (exon 3) siRNA to perform shRNA gene silencing Mouse - RGC-5 Syn G (Exon 3)

Get tips on using Human Ubiquitin/Ubiquitin+1 Antibody to perform Western blotting Ubiquitin

Get tips on using Anti-Beclin 1 (Human) pAb to perform Autophagy assay cell type - UMR-106

Get tips on using Human TGF beta 1 ELISA Kit (ab100647) to perform ELISA Human - TGF-beta 1

Get tips on using Human TGF-beta 1 Quantikine ELISA Kit to perform ELISA Human - TGF-beta 1

Get tips on using Human IL-1 beta ELISA Kit (ab100562) to perform ELISA Human - IL-1 beta

Get tips on using Human SDC1 / Syndecan-1 ELISA Kit to perform ELISA Human - SDC1

Get tips on using Human MCP-1 ELISA Kit (ab179886) to perform ELISA Human - MCP1

Get tips on using IRF-1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 IRF1

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.